Composite
Chelater

Part:BBa_K2664006:Design

Designed by: Areti-Efremia Mellou   Group: iGEM18_Macquarie_Australia   (2018-10-09)


Mg chelatase plasmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1720
    Illegal NotI site found at 3751
    Illegal NotI site found at 9504
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BglII site found at 304
    Illegal BglII site found at 960
    Illegal BglII site found at 1155
    Illegal BglII site found at 4456
    Illegal BglII site found at 4580
    Illegal BglII site found at 6580
    Illegal BamHI site found at 352
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3031
    Illegal NgoMIV site found at 3850
    Illegal NgoMIV site found at 4392
    Illegal NgoMIV site found at 5138
    Illegal NgoMIV site found at 6804
    Illegal AgeI site found at 5784
    Illegal AgeI site found at 7302
    Illegal AgeI site found at 7356
    Illegal AgeI site found at 7575
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 6906
    Illegal BsaI.rc site found at 7701
    Illegal SapI.rc site found at 7631


Design Notes

Codon optimisation, order of parts in operon, change of plac promoter to trc from previous parts


Source

Genes sourced from Chlamydomonas reinhardtii

References